Composite genomes of Ca. Actinomarinales Genomes

Composite genomes (CGs) of Ca. Actinomarina were reconstructed by co-assembly of groups of SAGs. To minimize possible bias, (i) only groups of genomes with an ANI >99% were used; (ii) these groups should have at least five representatives; and (iii) all regions of the genome must be covered by at least two fragments. Following this approach, three CGs belonging to genomospecies G1.1, G2.1, and G2.2 were reconstructed.The high degree of synteny and completeness of SAGs, the presence of contigs linking the beginning and the end of the genome, as well as the analysis of the GC skew give veracity to these genomes. Short of getting pure cultures, this is a good approach to reconstruct genomes of uncultivated microbes with realistic gene order and completion.