Density of IRS1 immunostaining in paired colonic epithelium, primary colorectal cancer and synchronous liver metastasis.

Density values (mean ± SEM), normalized per area size in square pixels, were obtained by digital analysis using ImageJ software (http://rsbweb.nih.gov/ij/) for IRS1 staining in: bottom versus top colonic crypt epithelium (respectively 11 and 10 areas); total colonic epithelium (78 areas) versus primary CRC (84 areas); total colonic epithelium (78 areas) versus liver metastasis (84 areas); primary CRC versus liver metastasis (84 areas). Only the differences between total colonic epithelium and liver metastasis and between primary CRC and liver metastasis are significant (P*Number of examined areas, CRC: colorectal cancer.