Qualitative and quantitative analysis of ROS–mediated oridonin-induced oesophageal cancer KYSE-150 cell apoptosis by atomic force microscopy.

ROS scavenger-NAC reversed oridonin induced cell cycle arrest in oesophageal cancer KYSE-150 cells (Fig A). Effects of oridonin on the adhesion force of KYSE-150 cells (Fig B). Typical force curve obtained on living KYSE-150 cells (Fig C). ROS scavenger-NAC reversed oridonin induced changes of Young’s modulus in fixed oesophageal cancer KYSE-150 cells (Fig D). ROS scavenger-NAC reversed oridonin induced cytoskeleton F-actin disruption in oesophageal cancer KYSE-150 cells (Fig E). (DOC)