Epithelial miR-141 regulates IL-13-induced airway mucus production [single-cell RNA-seq]. Epithelial miR-141 regulates IL-13-induced airway mucus production [single-cell RNA-seq]

We performed single cell RNA sequencing on bronchial cells from human bronchoalveolar lavage fluid from 4 independent study participants who had asthma and who underwent lung segmental allergen challenge with diluent or allergen (either house duse mite or cat). The goal was to assess total mRNAs in single cell preparations of bronchoalveolar lavage cells. Overall design: Single cell RNA sequencing was performed on a total of 8 samples of airway epithelial (bronchial) brushings obtained at bronchoscopy from subjects (n=4); 4 samples of which were collected after diluent challenge, and 4 samples collected after allergen challenge (with either house dust mite or cat allergen)