Genome-wide occupancy of RORB in neuroblastoma cells.

Neuroblastoma (NB), a malignant embryonic tumor arising from primitive neural crest cells, accounts for more than 7% of malignancies and around 15% of cancer-related mortality in childhood. Better elucidating the mechanisms of tumorigenesis and aggressiveness is important for improving the therapeutic efficiencies of NB. To investigate the mechanisms underlying the tumor suppressive functions of RAR-related orphan receptor B (RORB), we employed the Illumina HiSeq X Ten as a discovery platform to analyze the genome-wide occupancy of RORB on target genes in human SK-N-BE(2) cells. The results showed that 1637 target genes were regulated by transcriptional regulator RORB, especially those involved in regulation of NF-?B signaling pathway. Furthermore, we validated the ChIP-seq results by real-time PCR with high identity. Overall, our results provided fundamental information about the genomic enrichment of RORB in human NB cells, and these findings will help us understand the pathogenesis of NB. Overall design: ChIP-seq assay of genome-wide occupancy of transcriptional factor RORB in neuroblastoma SK-N-BE(2) cells.