El-Desouky Ammar, Saskia A. Hogenhout, L. R. Nault (2011) CIL:25367, Maize mosaic virus, Peregrinus maidis, secretory cell. CIL. Dataset
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Aggregates of Maize mosaic virus (MMV, Rhabdoviridae) in cells of the salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence). In the salivary gland, MMV aggregates are found mainly in the cell periphery or intercellularly, in contrast to other tissues in the insect vector that accumulate mainly around the nuclei (red). N, nucleus.
Methods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP).
Associated references:
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.
Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.
玉米花叶病毒(MMV,拉布多病毒科)在传病毒昆虫Peregrinus maidis(叶蝉,半翅目,网翅蝽科)唾液腺细胞中的聚集情况。针对MMV的抗体识别出(绿色/黄色荧光)聚集。在唾液腺中,MMV的聚集主要位于细胞外围或细胞间,与昆虫传病毒的其他组织主要在细胞核周围(红色)积累形成鲜明对比。N,细胞核。
提供机构:
CIL



