Summary of the specificity of molecular methods used in the study to determine the sample serotype composition.

* – results of the molecular assays targeting this set of serotypes were used in the analysis of method sensitivity in the studyA – serotype-specific signal no more than 2 CT stronger for the matching lytA signal from the sample.B – insufficient assay specificity.C – sequence of full homology (no single nucleotide mismatch) with either the sequence published for S. pneumoniae or the sequence detected in S. pneumoniae control strain tested in the study.D – sequence included when fully homologous with either the sequence published for S. pneumoniae or with the sequence detected in S. pneumoniae control strain tested in the study and excluded when not fully homologous.E - absence of full homology with S. pneumoniae sequences.