Transcriptome analysis of the treeshrew infected with ZIKV GZ01

We analyzed the gene expression profile of the PBMC from -2dpi, 2dpi, and 6dpi ZIKV-infected treeshrew Overall design: Two treeshreews (duplicated) infected with10^5 PFU of ZIKV(GZ01 strain) by the subcutaneous (s.c.) route. Peripheral blood mononuclear cells (PBMCs) from 0, 2 and 6 dpi (two for each group) were isolated using Ficoll-Hypaque and total RNA were extracted by RNeasy mini kit (Qiagen, German) for global transcriptome analysis.