WGS sequencing. Zygosaccharomyces rouxii CBS 732

In a large-scale comparative genomics analysis of hemiascomycetes yeasts, the Genolevures Consortium partially sequenced the genomes of 13 yeasts, including that of Zygosaccharomyces rouxii strain CBS 732. For each species, they prepared a random genomic DNA library containing 3- to 5-kb inserts and performed single-pass sequencing (up to 1 kb) of both ends of the inserts, resulting in random sequence tags (RSTs). The Genolevures Consortium generated 4,934 RSTs, totaling 4.5 Mb and representing approximately 0.4X genome coverage, for Zygosaccharomyces rouxii. They assembled these sequences into contigs and annotated them based on sequential comparisons to DNA and protein sequences from Saccharomyces cerevisiae, protein sequences from other completely sequenced organisms, and protein sequences from all other organisms. The Zygosaccharomyces rouxii sequences are available in GenBank/EMBL/DDBJ under the accessions AL392203 to AL397138.