Photodamage on rehydrated cryosections of different mouse tissue types.

The repetitive irradiation was performed in the central part of the images. CARS (red), TPEF (green) and SHG (blue) signals were acquired. The total number of scans used to produce the damage is also reported. The TPEF images of the first and last scan are shown for comparison. All irradiations were performed using the same experimental settings and therefore the scan numbers can be compared. Histological H&E staining of consecutive sections is reported as reference to visualize the tissue structures. All experiments were performed with a total laser power of ∼52 mW in the sample.