Longitudinal DNA methylation in exhaustively passaged astrocytes

This DNA methylation dataset describes epigenomic changes in astrocytes culture in vitro with passaging, in the context of studies examining cellular aging patterns that are conserved in vivo and in vitro. Fetal astrocytes were derived from cerebral cortex. Cells were grown under normoxic conditions and exhaustively passaged until cellular senescence. Longitudinal DNA samples were collected throughout passaging and DNA methylation was measured using the Infinium HumanMethylationEPIC BeadChip. In addition, some samples were sorted by FACS using senescence markers prior to DNA extraction for additional DNA methylation measurements. Astrocytes were cultured and exhaustively passaged for 3 cell lines derived from one male fetal donor. Samples taken at passages 2 through 10 (total n = 27 samples) and DNA extracted. DNA was also extracted again from samples at passages 2, 6 and 10 after sorting by FACS. This included bulk, low beta-galactosidase expression, high beta-galactosidase expression, and senescent cell samples (total n = 23 samples). These experiments were also performed using a fourth cell line isolated from a female fetal donor (n = 13 samples). Bisulphite converted DNA from samples were hybridized to the Illumina Infinium HumanMethylationEPIC BeadChip.