Purpose: The goals of this study are to generate transcriptome profiling (RNA-seq) of AT2 cells with over expression of Atf3 and Thra. Methods: AT2 cell mRNA profiles of 5 or 6-week-old wild-type mice
Evi1 overexpressed in SKOV3 cells Overall design: Four samples with Evi1-Flag plasmid transiently overexpressed. Four controls with Flag-only plasmid transiently overexpressed.
1p-values were calculated using a hypergeometric test Distribution of genes across categories in the strain collection and the gene set selected through the signature-tagged overexpression screen.
The RPMI-8226 human multiple myeloma cell line was stably infected with either a validated shRNA against BMI1 or a control shRNA. RNA was prepared from these lines, +/- doxycycline induction and at va