Study on the Response of Paddy Soil Methanogenic Microbes and Their Activities to PAHs

The process of soil methane production is a complex process that is metabolized by a variety of prokaryotic microorganisms, and methanogens are an important functional flora that mediates this complex process. There are currently three known metabolic pathways for methanogenesis: the first is the H2/CO2 nutrient type, which uses hydrogen or formic acid as an electron donor, and formate is oxidized to CO2 by formate dehydrogenase, and CO2 and MFR (methyl furan) ) Combine to form a formyl group, which is reduced to methyl by H4MTP (tetrahydromethylpterin) and transferred to CoM (coenzyme M) to form a methyl-CoM complex, which is finally reduced by methyl-CoM reductase (Mcr) The methyl group is methane; the second is the acetic acid nutrient type. Acetic acid is the main intermediate product of anaerobic metabolism of microorganisms. Methanogens decompose acetic acid through the acetic acid reaction, oxidize the carboxyl group to CO2, and reduce the methyl group to methane; Species are methylotrophs, mainly including methanol, methyl-containing amines, methyl-containing sulfides, etc. The methyl compound transfers the methyl group to CoM (Coenzyme M) through an intermediate protein to form a methyl-CoM complex , Which is eventually reduced to methane.