Single cell gene expression profile of GVHD-maintaining CD4 T cells isolated from spleen, colon, skin, and small intestine

We used single cell RNA sequencing (scRNAseq) to analyze the transcriptional profiles of GVHD maintaining T cells in various GVHD target tissues TS1 Tg CD4 cells were isolated from spleen, colon, skin, and small intestines (lamina propria and intraepithelial lymphocytes) of GVHD mice 4 weeks post-transplantation using fluorescence-activated cell sorting. After sorting, cells from each tissue were labelled with a unique TotalSeq-A hashing antibody and analyzed using scRNAseq HTO ID for Replicate 1: ACCCACCAGTAAGAC: Spleen_1 GGTCGAGAGCATTCA: Skin_1 CTTGCCGCATGTCAT: Colon_1 AAAGCATTCTTCACG: IEL_1 CTTTGTCTTTGTGAG: LP_1 HTO ID for Replicate 2: TATGCTGCCACGGTA: Spleen_2 GAGTCTGCCAGTATC: Skin_2 TATAGAACGCCAGGC: Colon_2 TGCCTATGAAACAAG: IEL_2 CCGATTGTAACAGAC: LP_2