Text S1 - Versatility of Cooperative Transcriptional Activation: A Thermodynamical Modeling Analysis for Greater-Than-Additive and Less-Than-Additive Effects

Provides the following information: In Section 1 a rigorous mathematical derivation of the thermostatistical model (7) for multiple transcription factors is given. In Section 2 the model is compared with the thermostatistical model for transcription initiation proposed by Shea and Ackers. Section 3 provides mathematical details of the thermostatistical model for two activators. In Section 4 a proof is given that the thermostatistical modeling approach predicts that gene expression regulated by two activators is a monotonically increasing function of the activator concentrations. Section 5 provides various mathematical proofs necessary to show under which conditions less-than-additive and greater-than-additive effects are predicted by the thermostatistical two activator model for transcription initiation. (PDF)