Analysis of DNA methylation profile of Korean CLL patients with MBD-Seq

In this study, we performed genome-wide methylation profiling of eight CLL patients and five control subjects, in an Asian cohort. Methyl-CpG-binding domain sequencing (MBD-Seq) was used as the profiling method. Differential methylation analysis identified a number of differentially methylated regions (DMRs) and genes (DMGs). More regions were hypomethylated than were hypermethylated. Promoters contained the largest proportion of DMRs, while distal intergenic and intron regions contained the largest number of DMRs. Gene Ontology, pathway analysis, and network-based prioritization of DMGs were also performed.