Synthesis of novel fused acenaphtopyrimidine hybrid, its photophysical properties and HSA interaction

A facile and versatile procedure for the synthesis of novel functionalised 10-(1H-indol-3-yl)acenaphtho[1,2-<i>d</i>]pyrimidin-8-amine (compound <b>4</b>) has been described. The novel intermediate enone (<b>3</b>) and pyrimidine derivatives (<b>4</b>) were characterised by using FT-IR, NMR and MS. Moreover, the photophysical properties of compound <b>4</b> and its interaction with HSA (Human Serum Albumin) have been investigated by means of photometric and fluorimetric titration. Compound <b>4</b> showed the increase in fluorescence and hypsochromic shift of its maximum upon the addition of HSA that interaction is supported by molecular docking. Overall, Compound <b>4</b> has promising affinity towards HSA which was not previously reported for any fused acenaphtenone–pyrimidine hybrid analogues.