RNA-Sequencing of cardiac and renal tissues from CLP-induced mice and melatonin-treated mice with CLP

Melatonin (N-acetyl-5-methoxytryptamine) has been shown to have a cardioprotective effect against inflammatory diseases. However, the mechanisms underlying the protective role of melatonin in these diseases remain to be revealed. In this study, melatonin was administrated to mice 14 days before cecal ligation puncture surgery. The characteristics of sepsis-induced AKI and myocarditis were detected. The results showed that melatonin could alleviated cardiac and renal dysfunction in sepsis model. RNA-seq analysis showed that MLT repressed the oxidant stress in response to kidney injury. RNA sequencing assays with heart tissues showed melatonin maintains the mitochondrial function in sepsis-caused myocarditis. Overall design: Examination of 2 different cardiac and renal tissues from CLP-induced mice and melatonin-treated mice with CLP