Quercetin-loaded magnetic nanoparticles: A promising tool for antitumor treatment in human breast cancer cells

Quercetin (QUE) is a phytoestrogen with known antitumor properties; however, its hydrophobic nature and low bioavailability limit its efficacy as an anticancer drug. To address this, we explored loading QUE onto a non-toxic nanocarrier. This study focused on the biological activity of magnetic iron oxide nanoparticles coated with polyethylene glycol (MAG@PEG) loaded with QUE (MAG@PEG@QUE) in MCF-7 cells. The MAG@PEG nanosystem was synthesized using a hydrothermal method, and QUE was incorporated by adding an alcoholic solution of QUE to an aqueous dispersion of MAG@PEG. QUE incorporation was confirmed qualitatively by FTIR spectroscopy and quantitatively through UV-visible spectroscopy. Cytotoxicity studies showed that MAG@PEG@QUE, at a concentration equivalent to the IC50 of free QUE, significantly reduced cell proliferation and viability while increasing apoptosis. MCF-7 cells treated with MAG@PEG@QUE also displayed actin cytoskeleton alterations typical of apoptotic cells. Transmission electron microscopy revealed clusters of magnetic nanoparticles within cellular vesicles. Targeted delivery of these nanoparticles was achieved using a static magnetic field, leading to high intracellular accumulation and selective cell death in targeted areas, without affecting adjacent cells. In conclusion, MAG@PEG@QUE shows comparable antitumor effects to free QUE and has the potential to enhance QUE’s bioavailability and targeted delivery for breast cancer treatment.