Enhanced DNA repair and genomic stability identify a novel HIV related Diffuse Large B-cell Lymphoma subtype [nanostring]. Enhanced DNA repair and genomic stability identify a novel HIV related Diffuse Large B-cell Lymphoma subtype [nanostring]

Transcriptional and genomic profiling study of HIV positive and HIV negative Diffuse Large B-Cell Lymphoma (DLBCL). Tumors were subtyped using the Lymph2Cx assay. Only GCB (germinal center like B-cell) subtypes were then subjected to digitial gene expression profiling and array comparative genomic hybridization (aCGH). The study used total RNA extracted from FFPE DLBCL tumors derived from HIV(+) and HIV(-) patients to assess differential expression of known cancer genes. Gene expression was correlated with IHC and array CGH data. Overall design: Digital gene expression profiling of 739 cancer related genes was performed on 19 HIV positive and 21 HIV negative GCB-DLBCL samples using the PanCancer Pathways panel and nCounter Technology from NanoString with customized spike-ins. DLBCL is more agressive in HIV(+) patients compared to HIV(-) individuals. Study objective was to identify novel markers and potential therapeutic targets that contribute to the enhanced agressive nature of this disease in HIV infected individuals.