Expression profile of Arabidopsis pistil/silique upon induction of FRUITFULL. Arabidopsis thaliana

To determine the genes that are regulated by FRUITFULL (FUL) in the pistil/silique, we identied differentially expressed genes after induction of FRUITFULL in ful-1 FUL-GR lines using the AGRONOMICS1 tiling array. The inflorescences of flowering plants were dipped in 10 uM DEX solution or in Mock Solution, and the flowers/siliques from stages 12-16 were harvested after 8 hours. RNA was extracted and DNase treated, and subsequent steps and hybridization to the AGRONOMICS Tiling array were performed by the Functional Genomics Center Zurich. Overall design: We generated several control samples to be able to identify genes that were differentially regulated by the DEX alone treatment instead of by the induction of FUL. A considerable number of genes was found to respond to DEX (samples ful-1 DEX vs ful-1 Mock). To identify the genes that were actually differentially upregulated because of FUL, we compared the samples ful-1_FUL-GR_DEX and ful-1_FUL_GR_Mock). All samples were performed in duplicate.