The role of aquaporin-3 (AQP3) in functions of alveolar macrophage (AM) during ovalbumin (OVA)-induced asthma

We compared the gene expression profiles of AMs using microarray analysis between AMs from wild-type (WT) and AQP3-deficient (AQP3-/-) mice before and after OVA challenge RNA was extracted from AMs of WT and AQP3-/- mice before and after OVA challenge. We prepared 4-6 mice for each group and pooled their samples.