Srebf1-mediated fatty acid metabolism is crucial to sustain sugmented pigmentation SREBF1-mediated metabolic reprogramming through fatty acid metabolism sustains augmented melanogenesis

Methods: mRNA profiles of B16 mouse melanoma cell line during pigmentation induced by culturing at low density were generated by deep sequencing, in dublicate. Library preparation was done using trueseq 4000 for quencing on Illumina paired end sequencing. The sequence read that passed the quality filter were aligned with GRCm38 as reference genome. Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample and identified 18753 transcripts in the pigmenting B16 melanocytes from D3 to D7 using HTSeq tool. R-values from Corelation plot varies from 0.98-0.99 between two replicates for different days. Overall design: mRNA prolife of B16 melanocytes during transition from depigmented to pigmented state (D3-D7)