Kinetic parameters of E495-M and E495-M-C1 towards peptide FAGFA and protein α-casein.
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aThe Km and kcat/Km values of E495-M and E495-M-C1 to FAGFA were determined at 25°C with Feder's method [23]. The data shown are the mean of three repeats with standard errors ≤5%. bThe Km values of E495-M and E495-M-C1 to α-casein at 50°C were determined by non-linear fit analysis based on Michaelis–Menten equation shown in Fig. S3. kcat values were calculated with the formula kcat = Vmax/[E].
创建时间：
2012-04-16
