A novel strategy for regulating mRNA's degradation via interfering the AUF1's binding to mRNA [RIP-seq]

Purpose: To verify the relationship between AUF1 and tumor cells proliferation, we detected the function of AUF1 in Hela cells using RIP-seq analysis. Methods: Genomic alignment (version from UCSC genome browser) was using Tophat (version:2.0.13) to get uniquely mapping reads. p value <0.05 considered as significantly modulated, were retained for further analysis. This choice is motivated by the decision to maximize the sensitivity of this analysis, in order to perform a massive screening and identify candidate genes to be validated with a wider sample population with real-time PCR analysis Results: The quantity of gene expression was calculated by FPKM (Fragments Per Kilobase of transcript per Million fragments mapped).50% of the binding region of AUF1 was in the exon region, and others were mainly in the 3'-UTR regions (29.6%). AUF1's targeted genes were related to metabolic and single-organism processes. AUF1's targeted genes were mainly concentrated in the protein synthesis process of the endoplasmic reticulum and cancer pathway. Conclusions: AUF1 might be involved in the regulation of translation and participated in the regulation of tumorigenesis and development through its target genes. Overall design: Fragment of RNA binding to AUF1 antibody in Hela cells