Decoding the molecular, cellular, and functional heterogeneity of zebrafish intracardiac nervous system

In this project, we present a comprehensive taxonomy of the intracardiac nervous system (IcNS) utilizing single-cell RNA sequencing, anatomical studies, and electrophysiological techniques. Overall design: For single-cell sequencing experiments, 25 Tg(elavl3:eGFP (previously known as HuC:GFP)) of 5-month-old animal were used from both sexes for the first set and 25 Tg(elavl3:eGFP) of 11-month-old, and 10 Tg(nbt:DsRed) of 22-month-old animals from both sexes were used for the second set. Cells were dissociated sorted by FACS as described (Cosacak et al. 2019). The single-cell encapsulation and cDNA synthesis done by following 10X Genomics' workflows. The reads were aligned to zebrafish genome (GRChZ 11, v 105) was used for gene annotation and assigning reads to genes. Cellranger software version 6.1.2 was used to process the data.