Interferon Alpha Induces Multiple Cellular Proteins That Coordinately Suppress Hepadnaviral Covalently Closed Circular DNA Transcription

To identify the cellular genes that mediate IFN-a suppression of cccDNA transcription, the total intracellular RNA were extracted from untreated or recombinant chicken IFN-a (rChIFN-a)-treated cells, and mRNA sequencing (mRNA-Seq) assays were performed in triplicate. And we found that treatment of dstet5 cells with 10 ng/ml of rChIFN-a for 6 h altered the expression of more than 100 cellular genes by more than 2 folds. Overall design: mRNA sequencing (mRNA-Seq) assays were performed in triplicate for untreated or rChIFN-a-treated dstet5 cells