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Single cell RNAseq of small intestinal stromal cells

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NIAID Data Ecosystem2026-03-13 收录
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We used scRNAseq approaches (Smartseq2 and 10X Genomics) to investigate the diversity of small intestinal stromal cells. Small intestines from adult C57Bl/6 mice were harvested and digested with Liberase TL. Single, live, CD45neg, EpCAMneg, CD31neg, gp38+ cells were either sorted into 384-well plates (Smartseq2) or captured in an oil emulsion (10X Genomics). RNA was amplified, barcoded and sequenced.

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2022-07-20
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