Depletion of MALAT1-dependent WTAP reduces aggressiveness of hypoxic TNBC cells

Numerous studies have identified long non-coding RNAs (lncRNA) as critical players in regulating tumor progression and metastasis formation. Here, we show that MALAT1 in hypoxic conditions, positively regulates WTAP protein expression, which influences the response to hypoxia by favoring the transcription of the master regulators HIF1A and HIF1B. Furthermore,WTAP stimulates BC cell migratory ability and the expression of N-Cadherin and Vimentin, hallmarks of epithelial-to-mesenchymal transition (EMT) RNA samples from 2 biological replicates of ChIRP assays performed in MDA-MB-468 cells to recover lncMALAT1-interacting RNAs have been pulled and subjected to RNAseq, along with relative Input samples used as reference