Microscopic images and schematics illustrating processes of microenvironment sensing and cortical actomyosin partitioning in T cells

The all-terrain motility of lymphocytes in tissues and tissue-like gels is best described as amoeboid motility. For amoeboid motility, lymphocytes do not require specific biochemical or structural modifications to the surrounding extracellular matrix. Instead, they rely on changing shape and steric interactions with the microenvironment. However, the exact mechanism of amoeboid motility remains elusive. Here we report that septins participate in amoeboid motility of T cells, enabling the formation of F-actin and alpha-actinin-rich cortical rings at the sites of cell cortex-indenting collisions with the extracellular matrix. Cortical rings compartmentalize cells into chains of spherical segments that are spatially conformed to the available lumens, forming transient ‘hourglass’-shaped steric locks onto the surrounding collagen fibers. The steric lock facilitates pressure-driven peristaltic propulsion of cytosolic content by individually contracting cell segments. Our results suggest that..., , , # Microscopic images and schematics illustrating processes of microenvironment sensing and cortical actomyosin partitioning in T cells

[https://doi.org/10.5061/dryad.m905qfv7g](https://doi.org/10.5061/dryad.m905qfv7g)

Dataset contains all source files for imaging data used in the manuscript.

## Description of the data and file structure

Source files are organized by name, as used in the manuscript's illustrations. Additionally, each source file contains a short legend, describing cells, dyes, antibodies, or drugs used.

## Sharing/Access information

NA

## Code/Software

1. **Analysis of spatial co-localization and cross-correlation for the cellular components of cortical rings and cell surface-indenting collagen fibers in hCD4+ T cell**

**Ring\_Analysis.zip**

**Main Scripts:**
orientation\_alignment.m – step 1: aligning the ring plane to the coordinate system
quantification\_ring\_positions.m – step 2: plotting end saving quantification of the overlap between (a...