Transcription analysis of CBX3::ALK Fusion and EML4-ALK Fusion that drives ALK Activation

Kinase gene fusions are critical oncogenic drivers and key targets in precision oncology. Here, we report a previously undescribed out-of-frame CBX3::ALK fusion identified in metastatic melanoma. Remarkably, this fusion generates a biologically active ALK protein. In this study, we performed RNA-seq to compare the transcriptional profile of CBX3::ALK with the well-characterized EML4-ALK fusion. Our goal is to evaluate the similarities between these fusions and investigate potential therapeutic targets for CBX3::ALK. Overall design: Comparative transcriptomic analysis of NIH/3T3 and MeWo cell lines expressing CBX3-ALK or EML4-ALK fusion genes versus control vectors.