Mean, minimum and maximum of the ratio of forward to reverse rate constants based on parameter sets resulting in hyperbolic and sigmoidal signal-response relationship.

The results shown are for topologies 14 and 30, assuming monofunctional HK, and sampling all parameters (with equal total protein concentrations at different layers). For additional results using alternative classification and sampling schemes (different total protein concentrations at different layers), assuming bifunctional HK, as well as for results from topologies 16 and 32, see Supporting Text S4. Mean values of all parameters as found in parameter sets resulting in hyperbolic and sigmoidal signal-response relationships in topologies 14, 16, 30 and 32 are provided as Supporting Text S5.