Individual nucleotide resolution Crosslinking Immunoprecipitation (iCLIP)-seq
收藏NIAID Data Ecosystem2026-04-30 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP220435
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Purpose: to identify the AGO1-bound miRNAs and mRNAs in human endothelial cells subject to hypoxic condition (2% oxygen) Methods: human microvascular endothelial cells was cultured under normoxia (20% oxygen) or hypoxia (2%) oxygen for 24 hours. Total RNA was extracted and RNA and small RNA libraries were prepared for Seq. Results: We found substantial changes in AGO1-bound mRNAs due to hypoxia. A portion of CLIP-seq reads also reveal direct binding to miRNA to mRNA. Conclusions: hypoxia induces profound changes in mRNAs associated with AGO1. Overall design: Biological replicates for each condition
创建时间：
2022-07-06
