Construction and development of new rabiesvirus vectors carrying enhanced greenfluorescent protein: supplementary data

Aim: Rabies is one of the most lethal viral diseases. This study developed a recombinant rabies virus,PVEGFP, expressing an EGFP protein based on PV strain to be applied in virus-neutralizing antibody (VNA)titration methods. Methods: The EGFP gene was inserted into the PVMCS strain, a previously discussedrecombinant virus. The recombinant virus was applied in the rapid fluorescent focus inhibition test (RFFIT)VNA titration method, and six vaccinated persons were studied for validation. Results: The recombinantrabies strain showed similar propagation properties to its ancestry strain, PVMCS. It could also measureVNA titers in the RFFIT method not significantly different from those acquired using the CVS-11 virus.Conclusion: Utilizing the PVEGFP strain in the RFFIT method can produce rapid, reliable and affordableresults.