MouseLight Neuron AL0002

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions. Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long. Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens. The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application at https://ml-neuronbrowser.janelia.org/. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.

一群来自Janelia的科学工作者发起了庞大的神经追踪工作，将小鼠大脑中完全追踪的神经元数量提升了十倍。研究者们现在可以下载并浏览三维空间中的数据。在小鼠大脑内部，单个神经元穿梭于半球之间，编织出错综复杂的分支图案，研究人员最新发现，这些神经元甚至能够延伸出长达45厘米的细丝。科学家们能够通过迄今为止创建的小鼠大脑接线图的最详细版本，观察并探索这些游走的神经轨迹。该图谱重建了小鼠大脑中7000万个神经元中超过300万个的形状和位置。以往追踪单个神经元路径的努力最多只能达到数十个。这些选择性标记的神经元通过迭代过程和双光子显微镜进行测绘。大脑被切割成200微米的薄片，每次标记并成像的神经元数量仅有几十个。每一张成像的大脑可产生约50太字节的数据，其中包含的测绘神经元可通过NeuronBrowser应用程序进行浏览，访问链接为https://ml-neuronbrowser.janelia.org/。对完整原始数据集感兴趣的研究者，请与Janelia联系，讨论通过硬件传输获取数据的方式。

搜集汇总

背景与挑战

背景概述

MouseLight Neuron AL0002数据集提供了小鼠大脑中单个神经元的三维追踪数据，包含SWC和JSON格式文件，用于神经科学研究。该数据集是Janelia科学家团队创建的详细小鼠大脑神经连接图的一部分，重建了300多个神经元的结构和位置。