M. agalactiae NCRs identified by high-throughput screening with host cells.

aNCRs were labeled with a letter based on their position in the genome of M. agalactiae.bLetters E and F indicate NCRs carrying insertions in mutants with reduced growth capacities on TIGMEC and/or TIGEF, respectively. Asterisks (*) indicate mutants that were also selected during high-throughput screening on HeLa cells.cFor each NCR, the number of mutants identified during the screening with caprine cells is indicated, as well as the number of insertion sites.dTransposon insertion sites were determined by direct sequencing of genomic DNA and their positions were defined based on the published genome sequence (NC_009497). The orientation of the transposon is indicated in parentheses.eSurrounding CDS and mini-Tn disrupted AMIGene CDS predictions; CDS names are given in parenthesis; vestige of ICEA indicates an NCR located within a 20 kb locus containing a vestige of an integrative conjugative element (ICEA).