Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Toe1 konckout Transcriptomes in differentiated C17.2 cells. Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Toe1 konckout Transcriptomes in differentiated C17.2 cells

We extracted RNA from NC and KO-Toe1 differentiated C17.2 cells for high-throughput transcriptomic sequencing. In differentiated C17.2 cells, Toe1 primarily affected biological functions including peptidase activity, chemotactic factors, the ECM, and the TNF signaling pathway. Overall design: mRNA profiles of NC and KO-Toe1 were generated by deep sequencing using Illumina GAIIx.