Transformation with oligos creating mismatches at 3′ termini.

For each genotype, the average of the ratio of the number of colonies obtained with a primer creating a 3′ mismatch to a primer with an internal mismatch (Trpwt40) is shown. Note that because the efficiency of transformation with Trpwt40 varies with orientation (it anneals to the lagging strand in strains of the F orientation), one cannot compare oligo transformation of F to R strains in the above table. Note that all strains are diploid; msh6/msh6 pol3–5DV/POL3 is represented as msh6 pol3-5DV±. There was not a significant number of transformants obtained with Oligo 148G in the trp5-G148T strains.
**Indicates a significant difference in transformation between msh6 pol3–5DV and msh6 pol3–5DV± strains as determined by a Mann-Whitney rank sum test.
* Indicates a significant difference in transformation between msh6 pol3–5DV and msh6 strains.
A 0 in the table indicates that no revertants were observed;
0.00 indicates that revertants were observed, but fewer than 1/100 of the number seen in the internal control transformation.
Transformation with oligos creating mismatches at 3′ termini.