Analysis of metabolites produced by D.fulvum

UPLC analysis of the metabolites produced by the soil bacterium D. fulvum when cultured in GYM media at different pH.

Samples were analyzed by analytical UPLC. Analytical (non-high resolution) spectrometry analysis (UHPLC-MS) were performed on an Ultimate 3000 UHPLC system, coupled with a LCQ Fleet Ion Trap Mass Spectrometer (Thermo Scientific). Chromatographic separation was achieved using an Acquity UPLC Peptide BEH C18 Column (300Å, 1.7 µm, 2.1 mm X 100 mm). Mobile phase system was composed of solvent A (H2O, 0.1% formic acid) and B (acetonitrile, 0.1% formic acid), typically run through a linear gradient from 0% to 100% of solvent B in 10 min. Elution of compounds was monitored by UV absorbance at 215 nm and 254 nm, and by mass spectrometry by electrospray ionization.