Biallelic TET2 mutation sensitizes to 5’-azacitidine in acute myeloid leukemia. Stolzel et al. JCI Insight. [RNA-Seq]

To investigate the impact of TET2 loss on global transcription in HEL acute myeloid leukaemia (AML) cells. HEL AML cells, which have a monoallelic TET2 mutation, were targeted by CRISPR to inactivate the remaining TET2 allele, generating isogenic cell clones with either monoallelic or biallelic TET2 mutation. We then performed comparative gene expression profiling analysis using data obtained from RNA-seq of 3 indepenent cell clones with monoallelic TET2 mutation and 3 independent cell clones with biallelic TET2 mutation.