Unseeded saPMCA experiments carried out in prion free laboratory.

Footnote to Table 1. Eleven independent experiments were carried out in the prion free laboratory using 4 to 6 replicates of unseeded substrate, in absence of any positive inoculum. This table provides details on each experiment reporting the number substrates samples, the PrP genotype and the age of the individual voles. Animals belong to four classes of age: a = 4–8 weeks old; b = 2–4 months old; c = 5–12 months old; d =  older than 1 year. The settings of saPMCA (the length of incubation and sonication cycles, the power of sonication, number of consecutive rounds and the length of rounds) are also given.