Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:35149, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 by RNAi expressing H2B::GFP by DIC and fluorescence microscopy. GFP-tagged histone H2B allows chromosome segregation to be followed. In wild-type, DNA replication generates two sets of sister chromatids that segregate toward opposite spindle poles during mitosis. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. In npl-4(RNAi) embryos, the movie shows chromosome bridges during mitosis as well as a delay in chromatin condensation, especially in the P1 cell. The series corresponds to experiments shown in Fig. 3A and Fig S5 and Movie 8 of Mouysset et al. Movie is CIL 25635 and original data is CIL35149.

本时序影像系列展现了C. elegans胚胎在npl-4基因通过RNA干扰表达H2B::GFP后，早期细胞分裂直至四细胞阶段的动态过程。通过差分干涉显微镜和荧光显微镜观察到，GFP标记的组蛋白H2B允许我们追踪染色体的分离。在野生型中，DNA复制产生两套姐妹染色单体，在有丝分裂过程中向相反的纺锤体两极分离。P0合子的第一次C. elegans胚胎分裂是不对称的，生成前端的AB细胞和较小的后端P1细胞。这些细胞具有不同的发育命运和分裂时间，AB细胞分裂大约在P1细胞之前2分钟。在npl-4(RNAi)胚胎中，影片展示了有丝分裂过程中的染色体桥以及染色质凝缩的延迟，特别是在P1细胞中。本系列与Mouysset等人的图3A、图S5和电影8中的实验相对应。电影编号为CIL 25635，原始数据编号为CIL35149。