Formate supplementation enhances anti-tumor CD8+ T cell fitness and efficacy of PD-1 blockade

Metabolic support of the response to immune checkpoint blockage is unknown. We used scRNA-seq to determine the response of CD8+ cells to metabolic supplementation with the one carbon donor formate in combination with anti-PD-1 Tumor infiltrating CD8+ cells were purified by FACS on day 12 post implantation from B16-OVA tumors after treatment with formate (starting day 9) and antibody (starting day 10). Four treatment groups, control drinking water with isotype control antibody (C.I.), formate drinking water with isotype control antibody (F.I.), control drinking water with anti-PD-1 antibody (C.P.), and formate drinking water with anti-PD-1 antibody (F.P.).