Vela or Imig activities were assayed as described in Methods. The t½ was the time at which GCase activity decreased to 50% of initial values. n = 3 to 6 for all assays.
The standard error (SE) and t values of the parameters are shown.aEnzyme activities were log-transformed.bLower andcupper 95% confidence limits for each estimates (10,000 iterations).
Hydrolytic activity values, with the corresponding turnover rates, determined for native SERCA, native Na,K-ATPase and recombinant SERCA (WT) at 37°C. In the case of SERCA, the presence or absence of
Vela or Imig activities were assayed as described in Methods. The t½ was the time at which GCase activity decreased to 50% of initial values. n = 3 to 6 for all assays.
