Nuclear receptor expression in lung tissue after vaping exposure

The emerging concern about chemicals in electronic cigarettes, even those without nicotine, de-mands the development of advanced criteria for their exposure and risk assessment. This study aims to highlight the sensitivity of lung nuclear receptors (NRs) to electronic cigarette e-liquids, independent of nicotine presence, and the influence of the sex variable on these effects. Adult male and female C57BL/6J mice were exposed to electronic cigarettes with 0%, 3% and 6% nicotine daily (70 ml, 3.3s, 1 puff per min/30 min) for 14 days, using the inExpose full body chamber (SCIREQ). Following exposure, lung tissue was harvested and RNA extracted. The expression of 84 NRs was determined using the RT2 profiler mRNA array (QIAGEN).