Genome-wide SF-1 (NR5A1) binding sites in NCI-H295R cells. Homo sapiens

The nuclear receptor steroidogenic factor-1 (SF-1, NR5A1) is a key regulator of adrenal and gonadal biology. We aimed to identify a novel subset of SF-1 target genes in the adrenal by performing ChIP-on-chip in NCI-H295R human adrenocortical cells using promoter tiling arrays. Analysis of ChIP-on-chip experiments with CisGenome identified 738 SF-1-binding regions that met criteria of an MA score more than 3.5 mean ± S.D. and a false discovery rate of 3.0) Overall design: Unstimulated NCI-H295R cells ChIPed with Upstate Millipore 07-618 anti-SF-1 antibody vs input chromatin. Two full experiments were analysed (biological replicates): 2 SF-1 ChIP arrays, 2 input control arrays