Expression data from E12.5 male fatal PGCs, PGCLC in vitro, round spermatids and spermatids like cells produced in vitro

Generation of haploid gametes in vitro can potentially address gamete failure-based infertility.This study reports complete in vitro meiosis from murine ESC-derived PGCLCs resulting in the formation of male spermatid-like cells (SLCs) capable of producing viable fertile offspring via intracytoplasmic sperm injection (ICSI).Our findings provide the basis for generation of haploid spermatids in vitro in human, the generation of transgenic animals, and the use of this system to investigate mechanisms of meiosis. We used microarrays to compare gene expression profiles of in vivo and in vitro derived PGC cells and round spermatids. We collected E12.5 male fatal PGCs, PGCLC in vitro, round spermatids and spermatids like cells produced in vitro, each sample has 3 replications.体外单倍体配子（haploid gametes）的生成，有望解决因配子功能异常引发的不孕症。本研究实现了从小鼠胚胎干细胞（ESC）诱导的原始生殖细胞样细胞（PGCLCs）出发的完全体外减数分裂，获得了雄性精子细胞样细胞（SLCs）；此类细胞可通过卵胞浆内单精子注射（ICSI）产生具有存活能力且可育的后代。本研究结果为人类体外单倍体精子细胞的生成、转基因动物的构建，以及利用该体系探究减数分裂机制提供了理论基础。我们采用基因芯片（microarrays）比较了体内来源与体外来源的原始生殖细胞（PGC）以及圆形精子细胞的基因表达谱。我们收集了E12.5天雄性胎鼠原始生殖细胞、体外诱导的原始生殖细胞样细胞、圆形精子细胞以及体外生成的精子细胞样细胞，每个样本设置3次生物学重复。