Inflammation and senescence-related microRNAs from circulating small extracellular vesicles are altered in women undergoing preterm birth.

Preterm labor (PTL) and preterm premature rupture of membranes (PPROM) lead to severe perinatal morbidity/mortality worldwide. Small extracellular vesicles (sEV) act in cell communication and contain microRNAs that are potential biomarkers for these complications. We aimed to compare the expression, in sEV from peripheral blood, of miRNAs between term and preterm pregnancies. Case-control study with women with PTL, PPROM, term pregnancy with and without labor, seen at the Botucatu Medical School Hospital, SP, Brazil. sEV were isolated from plasma. Western blot for detection of exosomal protein CD63 and Nanoparticle Tracking Analysis were performed. The expression of 800 miRNAs was assessed by nCounter Humanv3 miRNA Assay (NanoString). Comparison of miRNAs expression and relative risk was performed.