Quantification of total RNA and protein.
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The concentration of total RNA was determined with the BioAnalyzer 2100 (Agilent Technologies) employing an Agilent RNA 6000 Nano Chip using the device setting “EukaryoteTotal RNA Nano assay”. Protein concentration was determined by the BCA-method (Pierce) according to the manufacturers' instructions and re-analysed densitometrically on an SDS-PAGE by comparison to different amounts of a BSA-standard; nd = not determined; * = estimated by silver staining; bs, bisexual; us, unisexual; mT, male tegument.
创建时间：
2013-07-25
