Cucurbita pepo cultivar:line X10 and line JIN234 Raw sequence reads. Cucurbita pepo cultivar:line X10 and line JIN234

Dwarfism is an important agronomic trait in pumpkin that could increase yield. In this study, the dwarf Cucurbita pepo L. line X10 exhibited significantly longitudinally shorter cells in the stem than the normal-vine line JIN234. The dwarf stature of X10 could be recovered with exogenous gibberellin (GA3) application, suggesting that X10 might be sensitive in GA biosynthesis. Genetic analysis revealed that this dwarf trait is controlled by a single incompletely dominant locus, CpDw (Cucurbita pepo L. Dwarf). Using 1,300 F2 individuals derived from a cross between X10 and JIN234, we mapped the CpDw locus to a region of approximately 24.6 kb on chromosome 10, which contained 5 annotated genes. Genetic and molecular analyses revealed that the GA 2-oxidase gene Cp4.1LG10g05910.1 is a possible candidate gene for CpDw. Further allelic diversity analysis in 60 accessions of Cucurbita spp. indicated that Cp4.1LG10g05910.1 may be involved in vine growth during the early developmental stage in C. pepo L., but not in C. maxima and C. moschata. This study provides an important theoretical basis for the genetic regulation of vine length and crop breeding in pumpkin.